Indeed, hydrogel-integrated fibrous scaffolds allow for cellular contact guidance within 3D environments, which cannot be accomplished with separate hydrogel or electrospun fiber systems. In conclusion, 7.5% gelatin scaffold is more beneficial to the proliferation and fibrogenic differentiation of hDPSCs. An advantage of combining fibrous scaffolds with hydrogels is that cells can easily migrate through 3D environments, such as the 3D environment of native ECM. The levels of Collagen I, α-SMA, Periostin and Fibronectin were also higher in the 7.5% gelatin scaffold than in the 15% gelatin scaffold (P < 0.05). On day 7, the cell number on the 7.5% gelatin scaffold was significantly higher than that on the 15% gelatin scaffold (P < 0.05). To mimic the nano-fibrous architecture, a few technologies have been developed to engineer nano-fibrous scaffolds. Numerous scaffold types, such as porous, hydrogel, fibrous, microsphere, metal, composite and decellularized matrix, have been. As an essential component of tissue engineering, scaffolds provide structural and functional support for cell growth and differentiation. Both 7.5% and 15% gelatin scaffolds could promote the adhesion and growth of hDPSCs. Collagen is a major natural extracellular matrix component, and possesses a fibrous structure with fiber bundles varying in diameter from 50-500 nm 124, 125. Over centuries, several advances have been made in osteochondral (OC) tissue engineering to regenerate more biomimetic tissue. In the 15% gelatin scaffold, fiber bonding was detected and strengthened until the emergence of flat structures after cross-linking. RESULTS AND CONCLUSION: The fiber diameter of the 7.5% gelatin scaffold was (2.02☐.36) μm, and it was increased to (3.15☐.52) μm after cross-linking. The human dental pulp stem cells (hDPSCs) were seeded on the scaffolds and the cell proliferation and fibrogenic differentiation were tested using MTT and RT-PCR. The surface morphology and physical properties of gelatin scaffolds were tested by using scanning electron microscope and tensile tests. METHODS: Gelatin scaffolds at different concentrations were prepared by electrospinning method. OBJECTIVE: To study whether a gelatin scaffold can induce dental pulp stem cells to differentiate into fibroblasts. The physicochemical properties and biocompatibility of scaffold materials are crucial for proliferation and differentiation of stem cells. Determining how to achieve dental pulp regeneration has become a research focus in dentistry. ABSTRACT BACKGROUND: A tooth can be led to lose viability, split easily and miss immune defensive response by pulpitis and pulp necrosis.
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